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Product Name
RHO Polyclonal Antibody
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Description
Polyclonal antibody to RHO
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Tested applications
WB, IHC
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Species reactivity
Mouse, Rat
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Alternative names
RHO antibody; CSNBAD1 antibody; OPN2 antibody; RP4 antibody; rhodopsin antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: A synthetic peptide corresponding to a sequence within amino acids 200 to the C-terminus of human RHO (NP_000530.1).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
IHC 1:50 - 1:200 -
Validations
Western blot - RHO Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using RHO antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 10s.
Immunohistochemistry - RHO Polyclonal Antibody
Immunohistochemistry of paraffin-embedded mouse retina using RHO antibody at dilution of 1:200 (40x lens).
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Background
Photoreceptor required for image-forming vision at low light intensity. Required for photoreceptor cell viability after birth. Light-induced isomerization of the chromophore 11-cis-retinal to all-trans-retinal triggers a conformational change that activates signaling via G-proteins. Subsequent receptor phosphorylation mediates displacement of the bound G-protein alpha subunit by the arrestin SAG and terminates signaling.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"