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Product Name
RFWD3 antibody
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Description
RFWD3 Rabbit Polyclonal antibody. Positive WB detected in mouse lung tissue. Positive IHC detected in human testis. Positive IF detected in Hela. Observed molecular weight by Western-blot: 85kd
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Tested applications
ELISA, WB, IHC, IF
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Species reactivity
human, mouse, rat; other species not tested.
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Alternative names
RFWD3 antibody; RNF201 antibody; RING finger protein 201 antibody; FLJ10520 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of RFWD3 recombinant protein (Accession Number: BC002574). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IHC: 1:20-1:200
IF: 1:20-1:200
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Validations
mouse lung tissue were subjected to SDS PAGE followed by western blot with Catalog No:114633(RFWD3 antibody) at dilution of 1:1000
Immunohistochemical of paraffin-embedded human testis using Catalog No:114633(RFWD3 antibody) at dilution of 1:400 (under 10x lens)
Immunohistochemical of paraffin-embedded human testis using Catalog No:114633(RFWD3 antibody) at dilution of 1:400 (under 40x lens)
Immunofluorescent analysis of Hela cells, using RFWD3 antibody Catalog No:114633 at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
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Background
RFWD3, also named as RNF201, is a E3 ubiquitin-protein ligase that mediates the ubiquitination of p53/TP53 in the late response to DNA damage, and acts as a positive regulator of p53/TP53 stability, thereby regulating the G1/S DNA damage checkpoint. RFWD3 may act by catalyzing the formation of short polyubiquitin chains on p53/TP53 that are not targeted to the proteasome. In response to ionizing radiation, It interacts with MDM2 and enhances p53/TP53 ubiquitination, possibly by restricting MDM2 from extending polyubiquitin chains on ubiquitinated p53/TP53.(PMID: 20173098 ) From BLAST result, this antibody is specific to RFWD3. 45kd and 55kd maybe splicesome of RFWD3.
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