RFK antibody

Cat.#: 114628

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Product Information

  • Product Name
    RFK antibody
  • Documents
  • Description
    RFK Rabbit Polyclonal antibody. Positive WB detected in HeLa cells, Jurkat cells. Positive IF detected in HeLa cells. Observed molecular weight by Western-blot: 18 kDa
  • Tested applications
    ELISA, WB, IF
  • Species reactivity
    Human,Mouse,Rat; other species not tested.
  • Alternative names
    Flavokinase antibody; FLJ11149 antibody; RFK antibody; riboflavin kinase antibody; RIFK antibody
  • Immunogen
  • Isotype
    Rabbit IgG
  • Preparation
    This antibody was obtained by immunization of RFK recombinant protein (Accession Number: BC007069). Purification method: Antigen affinity purified.
  • Clonality
    Polyclonal
  • Formulation
    PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
  • Storage instructions
    Store at -20℃. DO NOT ALIQUOT
  • Applications

    Recommended Dilution:

    WB: 1:500-1:5000

    IF: 1:10-1:100

  • Validations

    HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:114628(RFK antibody) at dilution of 1:500

    HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:114628(RFK antibody) at dilution of 1:500

    Immunofluorescent analysis of (-20oc Ethanol) fixed HeLa cells using Catalog No:114628(RFK Antibody) at dilution of 1:25 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)

    Immunofluorescent analysis of (-20oc Ethanol) fixed HeLa cells using Catalog No:114628(RFK Antibody) at dilution of 1:25 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)

  • Background
    Riboflavin kinase (RFK) is an essential enzyme that catalyzes the phosphorylation of riboflavin (vitamin B2) to form flavin mononucleotide (FMN), an obligatory step in vitamin B2 utilization and flavin cofactor synthesis(PMID:12623014). RFK is rate-limiting in the synthesis of FAD, an essential prosthetic group of NADPH oxidase.
  • References
    • Schramm M, Wiegmann K, Schramm S. Riboflavin (vitamin B2 ) deficiency impairs NADPH oxidase 2 (Nox2) priming and defense against Listeria monocytogenes. European journal of immunology. 44(3):728-41. 2014.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"