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Product Name
RFK antibody
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Description
RFK Rabbit Polyclonal antibody. Positive WB detected in HeLa cells, Jurkat cells. Positive IF detected in HeLa cells. Observed molecular weight by Western-blot: 18 kDa
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Tested applications
ELISA, WB, IF
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
Flavokinase antibody; FLJ11149 antibody; RFK antibody; riboflavin kinase antibody; RIFK antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of RFK recombinant protein (Accession Number: BC007069). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IF: 1:10-1:100
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Validations
HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:114628(RFK antibody) at dilution of 1:500
Immunofluorescent analysis of (-20oc Ethanol) fixed HeLa cells using Catalog No:114628(RFK Antibody) at dilution of 1:25 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Background
Riboflavin kinase (RFK) is an essential enzyme that catalyzes the phosphorylation of riboflavin (vitamin B2) to form flavin mononucleotide (FMN), an obligatory step in vitamin B2 utilization and flavin cofactor synthesis(PMID:12623014). RFK is rate-limiting in the synthesis of FAD, an essential prosthetic group of NADPH oxidase.
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References
- Schramm M, Wiegmann K, Schramm S. Riboflavin (vitamin B2 ) deficiency impairs NADPH oxidase 2 (Nox2) priming and defense against Listeria monocytogenes. European journal of immunology. 44(3):728-41. 2014.
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