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Product Name
RFC1 Polyclonal Antibody
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Description
Polyclonal antibody to RFC1
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Tested applications
WB, IF
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Species reactivity
Human
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Alternative names
RFC1 antibody; A1 antibody; MHCBFB antibody; PO-GA antibody; RECC1 antibody; RFC antibody; RFC140 antibody; replication factor C subunit 1 antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 400-700 of human RFC1 (NP_001191676.1).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
IF 1:50 - 1:200 -
Validations
Western blot - RFC1 Polyclonal Antibody
Western blot analysis of extracts of MCF-7 cells, using RFC1 antibody at 1:500 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.
Immunofluorescence - RFC1 Polyclonal Antibody
Immunofluorescence analysis of MCF-7 cells using RFC1 antibody . Blue: DAPI for nuclear staining.
Immunofluorescence - RFC1 Polyclonal Antibody
Immunofluorescence analysis of A549 cells using RFC1 antibody .
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Background
The elongation of primed DNA templates by DNA polymerase delta and epsilon requires the action of the accessory proteins PCNA and activator 1. This subunit binds to the primer-template junction. Binds the PO-B transcription element as well as other GA rich DNA sequences. Could play a role in DNA transcription regulation as well as DNA replication and/or repair. Can bind single- or double-stranded DNA.; Interacts with C-terminus of PCNA. 5' phosphate residue is required for binding of the N-terminal DNA-binding domain to duplex DNA, suggesting a role in recognition of non-primer template DNA structures during replication and/or repair.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"