REST Polyclonal Antibody

Cat.#: 165226

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Product Information

  • Product Name
    REST Polyclonal Antibody
  • Documents
  • Description
    Polyclonal antibody to REST
  • Tested applications
    WB
  • Species reactivity
    Human
  • Alternative names
    REST antibody; NRSF antibody; WT6 antibody; XBR antibody; RE1-silencing transcription factor antibody
  • Isotype
    Rabbit IgG
  • Preparation
    Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 760-1060 of human REST (NP_001180437.1).
  • Clonality
    Polyclonal
  • Formulation
    PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
  • Storage instructions
    Store at -20℃. Avoid freeze / thaw cycles.
  • Applications
    WB 1:500 - 1:2000
  • Validations

    Western blot - REST Polyclonal Antibody

    Western blot - REST Polyclonal Antibody

    Western blot analysis of extracts of various cell lines, using REST antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 90s.

  • Background
    Transcriptional repressor which binds neuron-restrictive silencer element (NRSE) and represses neuronal gene transcription in non-neuronal cells. Restricts the expression of neuronal genes by associating with two distinct corepressors, mSin3 and CoREST, which in turn recruit histone deacetylase to the promoters of REST-regulated genes. Mediates repression by recruiting the BHC complex at RE1/NRSE sites which acts by deacetylating and demethylating specific sites on histones, thereby acting as a chromatin modifier. Transcriptional repression by REST-CDYL via the recruitment of histone methyltransferase EHMT2 may be important in transformation suppression. Key repressor of gene expression in hypoxia; represses genes in hypoxia by direct binding to an RE1/NRSE site on their promoter regions. Negatively regulates the expression of SRRM3 in breast cancer cell lines. Represses the expression of SRRM4 in non-neural cells to prevent the activation of neural specific splicing events (By similarity). Acts as a regulator of osteoblast differentiation (By similarity).

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"