Rat Tie2?CD202b?/TEK (Fc Tag) recombinant protein

Rat Tie2?CD202b?/TEK (Fc Tag) recombinant protein

• DataSheet
• Material Safety Data Sheets (MSDS)

TEK, or TIE-2, is an endothelial cell-specific receptor tyrosine kinase (RTK) that is known as a functioning molecule of vascular endothelial cells. TEK comprises a subfamily of RTK with TIE, and these two receptors play critical roles in vascular maturation, maintenance of integrity and remodeling. Targeted mutagenesis of both Tek and its agonistic ligand, Angiopoietin-1, result in embryonic lethality, demonstrating that the signal transduction pathways mediated by this receptor are crucial for normal embryonic development. TEK signaling is indispensable for the development of the embryonic vasculature and suggests that TEK signaling may also be required for the development of the tumor vasculature.

Receptor protein-tyrosine kinase

CD202B; VMCM1; HYK; AA517024; TIE-2; TIE2; RP23-345A23.1; VMCM; STK1; TEK

D3ZCD0

Tek; rCG_53516

Human Cells

A DNA sequence encoding the rat TEK (NP_001099207.1) extracellular domain (Met 4-Leu 743) was fused with the Fc region of human IgG1 at the C-terminus.

Rat

The recombinant rat TEK/Fc is a disulfide-linked homodimer. The reduced monomer comprises 962 amino acids and predicts a molecular mass of 108 kDa. The apparent molecular mass of the rat TEK/Fc monomer is approximately 120-130 kDa in SDS-PAGE under reducing conditions due to glycosylation.

> 95 % as determined by SDS-PAGE

1. Measured by its binding ability in a functional ELISA.
2. Immobilized S1h-3C-mANGPT2 (Cat:501251) at 10 μg/mL (100 μL/well) can bind ratTEK-Fc.
The EC₅₀ of ratTEK-Fc is 0.26-0.62μg/mL.