Human Tie2?CD202b?/TEK (His Tag) recombinant protein

Human Tie2?CD202b?/TEK (His Tag) recombinant protein

• DataSheet
• Material Safety Data Sheets (MSDS)

TEK, or TIE-2, is an endothelial cell-specific receptor tyrosine kinase (RTK) that is known as a functioning molecule of vascular endothelial cells. TEK comprises a subfamily of RTK with TIE, and these two receptors play critical roles in vascular maturation, maintenance of integrity and remodeling. Targeted mutagenesis of both Tek and its agonistic ligand, Angiopoietin-1, result in embryonic lethality, demonstrating that the signal transduction pathways mediated by this receptor are crucial for normal embryonic development. TEK signaling is indispensable for the development of the embryonic vasculature and suggests that TEK signaling may also be required for the development of the tumor vasculature.

Angiopoietin-1 receptor

RP23-345A23.1; VMCM; STK1; TEK; VMCM1; HYK; CD202B; AA517024; TIE2; TIE-2

Q59HG2

TEK; TIE2; VMCM; VMCM1

Human Cells

A DNA sequence encoding the extracellular domain (Met 1-Lys 745) of human Tie2 (NP_000450.2) precursor was fused with a polyhistidine tag at the C-terminus.

Human

The recombinant human Tie2 comprises 734 amino acids and predicts a molecular mass of 82 kDa. As a result of glycosylation, the apparent molecular mass of rhTie2 is approximately 95-105 kDa in SDS-PAGE under reducing conditions.

> 95 % as determined by SDS-PAGE

Measured by its binding ability in a functional ELISA. Immobilized recombinant human Tie2 at 2 μg/ml (100 μl/well) can bind human Angiopoietin-2 at a linear range of 1.28-160 ng/ml.