Mouse EphA6 / EHK2 / Eph Receptor A6 (Fc Tag) recombinant protein

Mouse EphA6 / EHK2 / Eph Receptor A6 (Fc Tag) recombinant protein

• DataSheet
• Material Safety Data Sheets (MSDS)

Ephrin type-A receptor 6, also known as EphA6 or EHK2, belongs to the ephrin receptor subfamily of the protein-tyrosine kinase family which 16 known receptors (14 found in mammals) are involved: EPHA1, EPHA2, EPHA3, EPHA4, EPHA5, EPHA6, EPHA7, EPHA8, EPHA9, EPHA10, EPHB1, EPHB2, EPHB3, EPHB4, EPHB5, EPHB6. The Eph family of receptor tyrosine kinases (comprising EphA and EphB receptors) has been implicated in synapse formation and the regulation of synaptic function and plasticity6. Eph receptor−mediated signaling, which is triggered by ephrins7, probably modifies the properties of synapses during synaptic activation and remodeling. Ephrin receptors are components of cell signalling pathways involved in animal growth and development, forming the largest sub-family of receptor tyrosine kinases (RTKs). Ligand-mediated activation of Ephs induce various important downstream effects and Eph receptors have been studied for their potential roles in the development of cancer. In the vomeronasal system, Ephrin-A5/EphA6 interactions mediate attraction or adhesion rather than repulsion.

Receptor protein-tyrosine kinase

FLJ35246; M-EHK2; HEK12; DKFZP434C1418; EPHA6; EHK-2; EPA6; EK12; PRO57066; UNQ6114; EHK2

G1K381

Epha6

Human Cells

A DNA sequence encoding the extracellular domain of mouse EphA6 (NP_031964.2) (Met 1-Gln 546) was fused with the Fc region of human IgG1 at the C-terminus.

Mouse

The recombinant mouse EphA6/Fc is a disulfide-linked homodimer. The reduced monomer consists of 760 amino acids and has a predicted molecular mass of 85 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rm EphA6/Fc monomer is approximately 110 kDa due to glycosylation.

> 90 % as determined by SDS-PAGE

Measured by its binding ability in a functional ELISA. Immobilized mouse EphrinA3 at 1 μg/ml (100 μl/well) can bind mouse EPHA6 / chimera with a linear ranger of 6.25-400 ng/ml.