Rat EPHA3/Eph Receptor A3 (Fc Tag) recombinant protein

Rat EPHA3/Eph Receptor A3 (Fc Tag) recombinant protein

• DataSheet
• Material Safety Data Sheets (MSDS)

EPHA3 gene belongs to the ephrin receptor subfamily of the protein-tyrosine kinase family. EPH and EPH-related receptors have been implicated in mediating developmental events, particularly in the nervous system. The ephrin receptors are divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. EPHA3 gene encodes a protein that binds ephrin-A ligands. EPHA3 is involved in the retinotectal mapping of neurons. It may also control the segregation but not the guidance of motor and sensory axons during neuromuscular circuit development.

Receptor protein-tyrosine kinase

EPH-LIKE TYROSINE KINASE 1; HUMAN EMBRYO KINASE 1; EPHA3; EPH-LIKE KINASE 4; END3; MEK4; CEK4; EPHRIN TYPE-A RECEPTOR 3; ETK; HEK; TYROSINE-PROTEIN KINASE RECEPTOR ETK1; ETK1; AW492086; EK4; TYROSINE-PROTEIN KINASE TYRO4; HEK4; TYRO4 PROTEIN TYROSINE KINASE; EPH RECEPTOR A3; TYRO4

G3V9D5

Epha3; rCG_47005

Human Cells

A DNA sequence encoding the rat EPHA3 (EDL75897.1) (Met1-His541) was expressed with the Fc region of human IgG1 at the C-terminus.

Rat

The recombinant rat EPHA3/Fc is a disulfide-linked homodimer. The reduced monomer comprises 762 amino acids and has a predicted molecular mass of 85.8 kDa. The apparent molecular mass of the protein is approximately 103 kDa in SDS-PAGE under reducing conditions.

> 95 % as determined by SDS-PAGE

Measured by its binding ability in a functional ELISA.
Immobilized ratEFNA5-His (Cat:503443) at 10 μg/ml (100 μl/well) can bind rat EPHA3-Fc, The EC₅₀ of rat EPHA3-Fc is 12.1-28.4 ng/ml.