Rat EPHA3/Eph Receptor A3 (His Tag) recombinant protein

Rat EPHA3/Eph Receptor A3 (His Tag) recombinant protein

• DataSheet
• Material Safety Data Sheets (MSDS)

EPHA3 gene belongs to the ephrin receptor subfamily of the protein-tyrosine kinase family. EPH and EPH-related receptors have been implicated in mediating developmental events, particularly in the nervous system. The ephrin receptors are divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. EPHA3 gene encodes a protein that binds ephrin-A ligands. EPHA3 is involved in the retinotectal mapping of neurons. It may also control the segregation but not the guidance of motor and sensory axons during neuromuscular circuit development.

Receptor protein-tyrosine kinase

TYROSINE-PROTEIN KINASE TYRO4; TYRO4; EPH RECEPTOR A3; HEK4; TYRO4 PROTEIN TYROSINE KINASE; ETK; MEK4; CEK4; EPHRIN TYPE-A RECEPTOR 3; END3; EPH-LIKE KINASE 4; EPH-LIKE TYROSINE KINASE 1; HUMAN EMBRYO KINASE 1; EPHA3; EK4; TYROSINE-PROTEIN KINASE RECEPTOR ETK1; AW492086; ETK1; HEK

G3V9D5

Epha3; rCG_47005

Human Cells

A DNA sequence encoding the rat EPHA3 (EDL75897.1) (Met1-His541) was expressed with a polyhistidine tag at the C-terminus.

Rat

The recombinant rat EPHA3 comprises 532 amino acids and predicts a molecular mass of 61.2 kDa. The apparent molecular mass of the recombinant protein is approximately 70.5 kDa in SDS-PAGE under reducing conditions due to glycosylation.

> 90 % as determined by SDS-PAGE

Measured by its binding ability in a functional ELISA.
Immobilized rat EPHA3-His at 10 μg/ml (100 μl /well) can bind rat EFNA5-Fc(Cat:502973), The EC₅₀ of ratEFNA5-Fc (Cat:502973) is 9-20ng/ml.