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Product Name
RCN2 antibody
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Description
RCN2 Rabbit Polyclonal antibody. Positive IHC detected in human gliomas tissue, human testis tissue. Positive IF detected in HepG2 cells. Positive WB detected in human brain tissue, HEK-293 cells, mouse lung tissue. Positive IP detected in mouse lung tissue. Observed molecular weight by Western-blot: 55 kDa
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Tested applications
ELISA, WB, IHC, IF, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
Calcium binding protein ERC 55 antibody; E6 binding protein antibody; E6BP antibody; ERC 55 antibody; ERC55 antibody; RCN2 antibody; ReticulOCalbin 2 antibody; TCBP49 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of RCN2 recombinant protein (Accession Number: NM_002902). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IP: 1:200-1:1000
IHC: 1:20-1:200
IF: 1:20-1:200
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Validations
human brain tissue were subjected to SDS PAGE followed by western blot with Catalog No:114579(RCN2 antibody) at dilution of 1:400
Immunohistochemical of paraffin-embedded human gliomas using Catalog No:114579(RCN2 antibody) at dilution of 1:50 (under 10x lens)
Immunofluorescent analysis of HepG2 cells, using RCN2 antibody Catalog No:114579 at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).
IP Result of anti-RCN2 (IP:Catalog No:114579, 4ug; Detection:Catalog No:114579 1:400) with mouse lung tissue lysate 2800ug.
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Background
Reticulocalbin 2 (RCN2) is a Ca(2+)-binding protein that is specifically localized in the endoplasmic reticulum (ER). It is also termed ERC-55, i.e. ER calcium-binding protein of 55 kDa. It is a single copy gene and is encoded by an approximately 1900-base mRNA, which shows a ubiquitous expression pattern. The protein comprises an amino-terminal signal sequence followed by six copies of the EF-hand Ca2+ binding motif.
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References
- Li L, Sun L, Gao F. Stk40 links the pluripotency factor Oct4 to the Erk/MAPK pathway and controls extraembryonic endoderm differentiation. Proceedings of the National Academy of Sciences of the United States of America. 107(4):1402-7. 2010.
- Manichaikul A, Wang Q, Shi YL, Zhang Z, Leitinger N, Shi W. Characterization of Ath29, a major mouse atherosclerosis susceptibility locus, and identification of Rcn2 as a novel regulator of cytokine expression. American journal of physiology. Heart and circulatory physiology. 301(3):H1056-61. 2011.
- Yokoi N, Fukata Y, Kase D. Chemical corrector treatment ameliorates increased seizure susceptibility in a mouse model of familial epilepsy. Nature medicine. 21(1):19-26. 2015.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"