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Product Name
RCC1 antibody
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Description
RCC1 Rabbit Polyclonal antibody. Positive IHC detected in human testis tissue, human small intestine tissue. Positive IF detected in HepG2 cells. Positive WB detected in HeLa cells, HepG2 cells, mouse colon tissue, SH-SY5Y cells. Positive IP detected in HeLa cells. Observed molecular weight by Western-blot: 45-50 kDa,68 kDa
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Tested applications
ELISA, WB, IHC, IP, IF
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
Cell cycle regulatory protein antibody; CHC1 antibody; RCC1 antibody; RCC1 I antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of RCC1 recombinant protein (Accession Number: NM_001048199). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:1000-1:10000
IP: 1:500-1:5000
IHC: 1:20-1:200
IF: 1:20-1:200
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Validations
HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:114575(RCC1 antibody) at dilution of 1:1000
IP Result of anti-RCC1 (IP:Catalog No:114575, 3ug; Detection:Catalog No:114575 1:1500) with HeLa cells lysate 3000ug.
Immunohistochemical of paraffin-embedded human testis using Catalog No:114575(RCC1 antibody) at dilution of 1:50 (under 40x lens)
Immunofluorescent analysis of HepG2 cells using Catalog No:114575(RCC1 Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Background
CHC1, also named as RCC1, promotes the exchange of ran-bound gdp by gtp. It is involved in the regulation of onset of chromosome condensation in the S-phase. Phosphorylation of RCC1 on serines located in or near its nuclear localization signal activates RCC1 to generate RanGTP on mitotic chromosomes, which is required for spindle assembly and chromosome segregation. This antibody is a rabbit polyclonal antibody raised against full length RCC1 of human origin.
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