-
Product Name
RCAS1 antibody
- Documents
-
Description
RCAS1 Rabbit Polyclonal antibody. Positive WB detected in mouse heart tissue, HEK-293 cells, HeLa cells, human heart tissue. Positive IP detected in mouse heart tissue. Positive IF detected in HeLa cells. Positive IHC detected in human breast cancer tissue. Observed molecular weight by Western-blot: 32 kDa
-
Tested applications
ELISA, IHC, IP, IF, WB
-
Species reactivity
Human,Mouse,Rat; other species not tested.
-
Alternative names
EB9 antibody; EBAG9 antibody; PDAF antibody; RCAS1 antibody
-
Isotype
Rabbit IgG
-
Preparation
This antibody was obtained by immunization of RCAS1 recombinant protein (Accession Number: XM_047422412). Purification method: Antigen affinity purified.
-
Clonality
Polyclonal
-
Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
-
Storage instructions
Store at -20℃. DO NOT ALIQUOT
-
Applications
Recommended Dilution:
WB: 1:200-1:2000
IP: 1:200-1:2000
IHC: 1:20-1:200
IF: 1:10-1:100
-
Validations
Immunohistochemical of paraffin-embedded human breast cancer using Catalog No:114572(EBAG9 antibody) at dilution of 1:50 (under 10x lens)
mouse heart tissue were subjected to SDS PAGE followed by western blot with Catalog No:114572(EBAG9 Antibody) at dilution of 1:600
IP Result of anti-EBAG9 (IP:Catalog No:114572, 3ug; Detection:Catalog No:114572 1:800) with mouse heart tissue lysate 1000ug.
Immunofluorescent analysis of HeLa cells using Catalog No:114572(EBAG9 Antibody) at dilution of 1:25 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
-
Background
Estrogen receptor–binding fragment-associated antigen 9 (EBAG9) gene was identified as an estrogen-responsive gene. The gene product, receptor-binding cancer antigen expressed on SiSo cells (RCAS1), is associated with aggressive characteristics and poor overall survival for 15 different human malignancies. The correlation between RCAS1 expression and several clinicopathological variables, including tumor size, clinical stage, invasion depth and lymph node metastasis highlights this molecule's clinical significance. Expression of RCAS1 in tumor cells plays an important role in evasion from host immune system resulting tumor progression, invasion and metastasis. Further exploration of RCAS1 biological function will facilitate development of novel therapeutic strategies that target RCAS1.
Related Products / Services
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"