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Product Name
RBX1 antibody
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Description
RBX1 Rabbit Polyclonal antibody. Positive WB detected in HeLa cells, Jurkat cells. Positive IP detected in HeLa cells. Positive IHC detected in human heart tissue. Observed molecular weight by Western-blot: 12 kDa
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Tested applications
ELISA, WB, IHC, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
BA554C12.1 antibody; Protein ZYP antibody; RBX1 antibody; Regulator of cullins 1 antibody; ring box 1 antibody; RING box protein 1 antibody; RING finger protein 75 antibody; RNF75 antibody; ROC1 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of RBX1 recombinant protein (Accession Number: NM_014248). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:200-1:2000
IHC: 1:20-1:200
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Validations
HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:114568(RBX1 antibody) at dilution of 1:500
Immunohistochemical of paraffin-embedded human heart using Catalog No:114568(RBX1 antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemical of paraffin-embedded human heart using Catalog No:114568(RBX1 antibody) at dilution of 1:50 (under 40x lens)
IP Result of anti-RBX1 (IP:Catalog No:114568, 4ug; Detection:Catalog No:114568 1:500) with HeLa cells lysate 1520ug.
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Background
RBX1(RING-box protein 1) is also named as RNF75, Roc1 and is a requisite component of the multi- subunit SCF family E3s36–40.It mediates the ubiquitination and subsequent proteasomal degradation of target proteins, including proteins involved in cell cycle progression, signal transduction, transcription and transcription-coupled nucleotide excision repair.
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