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Product Name
RBPMS antibody
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Description
RBPMS Rabbit Polyclonal antibody. Positive IP detected in mouse heart tissue. Positive WB detected in mouse heart tissue, mouse kidney tissue, mouse liver tissue. Positive IF detected in Hela cells. Positive IHC detected in human ovary tumor tissue. Positive FC detected in HeLa cells. Observed molecular weight by Western-blot: 28-30 kDa
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Tested applications
ELISA, WB, IHC, IF, FC, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
HERMES antibody; RBP MS antibody; RBPMS antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of RBPMS recombinant protein (Accession Number: NM_001008712). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:200-1:1000
IHC: 1:20-1:200
IF: 1:10-1:100
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Validations
mouse heart tissue were subjected to SDS PAGE followed by western blot with Catalog No:114567(RBPMS antibody) at dilution of 1:400
Immunohistochemical of paraffin-embedded human ovary tumor using Catalog No:114567(RBPMS antibody) at dilution of 1:50 (under 40x lens)
Immunohistochemical of paraffin-embedded human ovary tumor using Catalog No:114567(RBPMS antibody) at dilution of 1:50 (under 10x lens)
Immunofluorescent analysis of Hela cells, using RBPMS antibody Catalog No:114567 at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
IP Result of anti-RBPMS (IP:Catalog No:114567, 4ug; Detection:Catalog No:114567 1:400) with mouse heart tissue lysate 4000ug.
1X10^6 HeLa cells were stained with 0.2ug RBPMS antibody (Catalog No:114567, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.
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Background
RBPMS represents RNA binding protein with multiple splicing
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"