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Product Name
RBM4 antibody
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Description
RBM4 Rabbit Polyclonal antibody. Positive IP detected in mouse heart tissue. Positive WB detected in mouse brain tissue, human brain tissue, human heart tissue, human kidney tissue. Positive IF detected in HepG2 cells. Positive IHC detected in human gliomas tissue, human lung cancer tissue. Observed molecular weight by Western-blot: 37-40 kDa
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Tested applications
ELISA, WB, IHC, IF, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
DKFZp547K0918 antibody; hLark antibody; LARK antibody; Lark homolog antibody; RBM4 antibody; RBM4A antibody; RNA binding motif protein 4 antibody; RNA binding motif protein 4a antibody; RNA binding protein 4 antibody; ZCCHC21 antibody; ZCRB3A antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of RBM4 recombinant protein (Accession Number: BC021120). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:500-1:5000
IHC: 1:20-1:200
IF: 1:20-1:200
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Validations
mouse brain tissue were subjected to SDS PAGE followed by western blot with Catalog No:114612(RBM4 antibody) at dilution of 1:500
Immunohistochemical of paraffin-embedded human gliomas using Catalog No:114612(RBM4 antibody) at dilution of 1:50 (under 40x lens)
Immunofluorescent analysis of HepG2 cells, using RBM4 antibody Catalog No:114612 at 1:100 dilution and FITC-labeled donkey anti-rabbit IgG(green). Blue pseudocolor = DAPI (fluorescent DNA dye).
IP Result of anti-RBM4 (IP:Catalog No:114612, 3ug; Detection:Catalog No:114612 1:1000) with mouse heart tissue lysate 4000ug.
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Background
RBM4, RNA-binding motif protein4, contains 2 RRM-type RNA-binding motif and a retroviral-type(RT) zinc finger. RNA-binding factor participates in number of aspects of cellular processes such as alternative splicing of pre-mRNA and translation regulation. RBM4 recruits eIF4A1 to stimulate IRES-dependent translation in responds to cellular stress. Once assembled at the rHRE, the HIF2A-RBM4-eIF4E2 complex captures the 5-prime cap and targets mRNAs to polysomes for active translation, thereby evading hypoxia-induced repression of protein synthesis. This is a rabbit polyclonal antibody raised against the full-length of human RBM4
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References
- Wang Y, Chen D, Qian H. The splicing factor RBM4 controls apoptosis, proliferation, and migration to suppress tumor progression. Cancer cell. 26(3):374-89. 2014.
- Markus MA, Marques FZ, Morris BJ. Resveratrol, by modulating RNA processing factor levels, can influence the alternative splicing of pre-mRNAs. PloS one. 6(12):e28926. 2011.
- Uniacke J, Holterman CE, Lachance G. An oxygen-regulated switch in the protein synthesis machinery. Nature. 486(7401):126-9. 2012.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"