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Product Name
RBM16 antibody
- Documents
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Description
RBM16 Rabbit Polyclonal antibody. Positive IP detected in HeLa cells. Positive WB detected in HT-1080 cells, A549 cells, HeLa cells, L02 cells, NIH/3T3 cells. Positive IF detected in Hela cells, HepG2 cells. Positive IHC detected in human testis tissue, human cervical cancer tissue. Observed molecular weight by Western-blot: 170 kDa
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Tested applications
ELISA, WB, IF, IHC, IP
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Species reactivity
Human, Mouse; other species not tested.
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Alternative names
CCAP7 antibody; KIAA1116 antibody; RBM16 antibody; RNA binding motif protein 16 antibody; RNA binding protein 16 antibody; SCAF8 antibody
- Immunogen
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of RBM16 recombinant protein (Accession Number: NM_014892). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:1000-1:10000
IP: 1:200-1:2000
IHC: 1:20-1:200
IF: 1:10-1:100
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Validations
HT-1080 cells were subjected to SDS PAGE followed by western blot with Catalog No:114601(RBM16 antibody) at dilution of 1:2000
Immunohistochemical of paraffin-embedded human testis using Catalog No:114601(RBM16 antibody) at dilution of 1:100 (under 40x lens)
Immunofluorescent analysis of Hela cells, using RBM16 antibody Catalog No:114601 at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
IP Result of anti-RBM16 (IP:Catalog No:114601, 4ug; Detection:Catalog No:114601 1:800) with HeLa cells lysate 3600ug.
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Background
RBM16 represents RNA binding motif protein 16
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