Cart 2

RBAP48 antibody

Cat.#: 107497

Size:

Special Price 239.0 USD

Availability: In Stock
- +

Add to cart to get an online quotation

Product Information

  • Product Name
    RBAP48 antibody
  • Documents
  • Description
    RBAP48 Mouse Monoclonal antibody. Positive WB detected in Hepg2 cells, HeLa cells. Positive IHC detected in human cervical cancer tissue, human testis tissue. Positive FC detected in HeLa cells. Observed molecular weight by Western-blot: 53kD
  • Tested applications
    ELISA, WB, IHC, FC
  • Species reactivity
    Human; other species not tested.
  • Alternative names
    Histone binding protein RBBP4 antibody
  • Isotype
    Mouse IgG2b
  • Preparation
    This antibody was obtained by immunization of RBAP48 recombinant protein (Accession Number: NM_005610). Purification method: Protein A purified.
  • Clonality
    Monoclonal
  • Formulation
    PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
  • Storage instructions
    Store at -20℃. DO NOT ALIQUOT
  • Applications

    Recommended Dilution:

    WB: 1:500-1:5000

    IHC: 1:20-1:200

  • Validations

    HepG2 cells were subjected to SDS PAGE followed by western blot with Catalog No:107497(RBBP4 antibody) at dilution of 1:1000

    HepG2 cells were subjected to SDS PAGE followed by western blot with Catalog No:107497(RBBP4 antibody) at dilution of 1:1000

    Immunohistochemical of paraffin-embedded human cervical cancer using Catalog No:107497(RBBP4 antibody) at dilution of 1:200 (under 10x lens)

    Immunohistochemical of paraffin-embedded human cervical cancer using Catalog No:107497(RBBP4 antibody) at dilution of 1:200 (under 10x lens)

    Immunohistochemical of paraffin-embedded human cervical cancer using Catalog No:107497(RBBP4 antibody) at dilution of 1:200 (under 40x lens)

    Immunohistochemical of paraffin-embedded human cervical cancer using Catalog No:107497(RBBP4 antibody) at dilution of 1:200 (under 40x lens)

    1X10^6 HeLa cells were stained with 0.2ug RBBP4 antibody (Catalog No:107497, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG(H+L) with dilution 1:1000.

    1X10^6 HeLa cells were stained with 0.2ug RBBP4 antibody (Catalog No:107497, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG(H+L) with dilution 1:1000.

  • Background
    Histone-binding protein RBBP4 (also known as RbAp48, or NURF55) is a protein that in humans is encoded by the RBBP4 gene. This gene encodes a ubiquitously expressed nuclear protein that belongs to a highly conserved subfamily of WD-repeat proteins. It is present in protein complexes involved in histone acetylation and chromatin assembly. It is part of the Mi-2/NuRD complex complex that has been implicated in chromatin remodeling and transcriptional repression associated with histone deacetylation. This encoded protein is also part of corepressor complexes, which is an integral component of transcriptional silencing. It is found among several cellular proteins that bind directly to retinoblastoma protein to regulate cell proliferation. A decrease of RbAp48 in the dentate gyrus (DG) of the hippocampus in the brain is suspected to be a main cause of memory loss in normal aging (PMID: 23986399).

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"