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Product Name
RB1CC1 antibody
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Description
RB1CC1 Rabbit Polyclonal antibody. Positive IF detected in HeLa cells. Positive IHC detected in human breast cancer tissue. Positive WB detected in MCF7 cells, HeLa cells, mouse skeletal muscle tissue, NIH/3T3 cells. Observed molecular weight by Western-blot: 180-200 kDa
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Tested applications
ELISA, WB, IHC, IF
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Species reactivity
Human, Mouse; other species not tested.
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Alternative names
CC1 antibody; DRAGOU14 antibody; FIP200 antibody; KIAA0203 antibody; RB1 inducible coiled coil 1 antibody; RB1CC1 antibody; RBICC antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of RB1CC1 recombinant protein (Accession Number: XM_011517643). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IHC: 1:20-1:200
IF: 1:10-1:100
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Validations
MCF7 cells were subjected to SDS PAGE followed by western blot with Catalog No:114587(RB1CC1 antibody) at dilution of 1:1000
Immunohistochemistry of paraffin-embedded human breast cancer slide using Catalog No:114587(RB1CC1 Antibody) at dilution of 1:50
Immunofluorescent analysis of HeLa cells using Catalog No:114587(RB1CC1 Antibody) at dilution of 1:25 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Background
RB1CC1, also named as RBICC, is implicated in the regulation of RB1 expression and functions as a DNA-binding transcription factor. It is a potent regulator of the RB1 pathway and a mediator that plays a crucial role in muscular differentiation. Its expression is, thus, a prerequisite for myogenic differentiation. Involved in autophagy. RB1CC1 is required for autophagosome formation. It is probably involved in the tumorigenesis of breast cancer. RB1CC1 is frequently mutated in breast cancer and shows characteristics of a classical tumor suppressor gene. This antibody is a rabbit polyclonal antibody raised against residues near the C terminus of human RB1CC1.
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