Rat VEGF-C (aa 108-223, His Tag) recombinant protein

Rat VEGF-C (aa 108-223, His Tag) recombinant protein

• DataSheet
• Material Safety Data Sheets (MSDS)

Vascular endothelial growth factor C (VEGF-C) is a member of the VEGF family. Upon biosynthesis, VEGF-C protein is secreted as a non-covalent momodimer in an anti-parellel fashion. VEGF-C protein is a dimeric glycoprotein, as a ligand for two receptors, VEGFR-3 (Flt4), and VEGFR-2. VEGF-C may function in angiogenesis of the venous and lymphatic vascular systems during embryogenesis. VEGF-C protein is over-expressed in various human cancers including breast cancer and prostate cancer. VEGF-C/VEGFR-3 axis, through different signaling pathways, plays a critical role in cancer progression by regulating different cellular functions, such as invasion, proliferation, and resistance to chemotherapy. Thus, targeting the VEGF-C/VEGFR-3 axis may be therapeutically significant for certain types of tumors.

Vascular endothelial growth factor C

O35757

Vegfc

Human Cells

A DNA sequence encoding the rat VEGFC (O35757) (Ala108-Arg223) was expressed, fused with a polyhistidine tag at the C-terminus.

Rat

The recombinant rat VEGFC comprises 127 amino acids and predicts a molecular mass of 14.5 kDa.

> 95 % as determined by SDS-PAGE

1. Immobilized rat VEGFC-His at 10 μg/mL (100 μL/well) can bind mouse VEGFR3-Fc (cat:502816), The EC₅₀ of mouse VEGFR3-Fc (cat:502816) is 17.4-40.6 ng/mL.
2. Measured in a cell proliferation assay using human umbilical vein endothelial cells (HUVEC). The ED₅₀ for this effect is 30-150ng/mL.