Human VEGF-C (His Tag) recombinant protein

Human VEGF-C (His Tag) recombinant protein

• DataSheet
• Material Safety Data Sheets (MSDS)

Growth factor active in angiogenesis, and endothelial cell growth, stimulating their proliferation and migration and also has effects on the permeability of blood vessels. May function in angiogenesis of the venous and lymphatic vascular systems during embryogenesis, and also in the maintenance of differentiated lymphatic endothelium in adults. Binds and activates VEGFR-2 (KDR/FLK1) and VEGFR-3 (FLT4) receptors.

Vascular endothelial growth factor C

VRP; LMPH1D; AW228853; VEGF-C; FLT4-L; VEGFC

P49767

VEGFC

Human Cells

A DNA sequence encoding the mature form of human VEGFC (NP_005420.1) corresponding to amino acid (Thr 103-Arg 227) was expressed with a C-terminal polyhistidine tag.

Human

The recombinant mature form of human VEGFC consists of 136 amino acids and has a predicted molecular mass of 15.5 kDa. In SDS-PAGE under reducing conditions, it migrates with an apparent molecular mass of 22-24 kDa due to glycosylation.

> 97 % as determined by SDS-PAGE

1. Measured by its binding ability in a functional ELISA.
Scatchard analysis showed the affinity constant (Kd) of recombinant human VEGF-C bound to recombinant human VEGFR3 was 1.4 nM.
2. Measured in a cell proliferation assay using human umbilical vein endothelial cells (HUVEC).
The ED₅₀ for this effect is 0.1-0.5μg/mL.