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Product Name
Rat BCAM / CD239 (His Tag) recombinant protein
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Description
The Lutheran (Lu) blood group and basal cell adhesion molecule (BCAM) antigens are both carried by 2 glycoprotein isoforms of the immunoglobulin superfamily representing receptors for the laminin alpha(5) chain. It is a transmembrane receptor with five immunoglobulin-like domains in its extracellular region, and is therefore classified as a member of the immunoglobulin (Ig) gene family. In addition to red blood cells, Lu/BCAM proteins are expressed in endothelial cells of vascular capillaries and in epithelial cells of several tissues. BCAM/LU has a wide tissue distribution with a predominant expression in the basal layer of the epithelium and the endothelium of blood vessel walls. As designated as CD239 recently, BCAM and LU share a significant sequence similarity with the CD146 (MUC18) and CD166, and themselves are adhesion molecules that bind laminin with high affinity. Laminins are found in all basement membranes and are involved in cell differentiation, adhesion, migration, and proliferation. BCAM is upregulated following malignant transformation of some cell types in vivo and in vitro, thus being a candidate molecule involved in tumor progression. In addition, BCAM interacts with integrin in sickle red cells, and thus may potentially play a role in vaso-occlusive episodes.
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Protein name
Basal cell adhesion molecule
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Protein short names
BCAM; LU; AU; GPLU; CD239; 1200005K12RIK; B-CAM; MSK19
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Uniprot ID
Q9ESS6
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Gene Name
Bcam; Lu
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Source/Expression Host
Human Cells
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Expression Plasmid/cDNA
A DNA sequence encoding the rat BCAM (Q9ESS6) (Met1-Ala543) was expressed, fused with a polyhistidine tag at the C-terminus.
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Protein Species
Rat
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Molecular weight
The recombinant rat BCAM comprises 529 amino acids and predicts a molecular mass of 58.2 kDa. The apparent molecular mass of the recombinant protein is approximately 68 kDa in SDS-PAGE under reducing conditions due to glycosylation.
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Purity
> 90 % as determined by SDS-PAGE
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Activity
Measured by the ability of the immobilized protein to support the adhesion of HOS human osteogenic sarcoma cells.
When cells are added to coated plates(10 μg/mL,100μL/well), approximately 20-50% will adhere for 1 hour incubation at 37℃. -
Validations
Rat BCAM Protein (His Tag) SDS-PAGE
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