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Product Name
RASGRF1 antibody
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Description
RASGRF1 Rabbit Polyclonal antibody. Positive IP detected in rat brain tissue. Positive WB detected in human brain tissue, rat brain tissue. Observed molecular weight by Western-blot: 130-140 kDa
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Tested applications
ELISA, WB, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
CDC25 antibody; CDC25L antibody; GNRP antibody; GRF1 antibody; GRF55 antibody; H GRF55 antibody; PP13187 antibody; Ras GRF1 antibody; RASGRF1 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of RASGRF1 recombinant protein (Accession Number: BC040275). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IP: 1:200-1:2000
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Validations
rat brain tissue were subjected to SDS PAGE followed by western blot with Catalog No:114477(RASGRF1 antibody) at dilution of 1:600
human brain tissue were subjected to SDS PAGE followed by western blot with Catalog No:114477(RASGRF1 antibody) at dilution of 1:600
IP Result of anti-RASGRF1 (IP:Catalog No:114477, 4ug; Detection:Catalog No:114477 1:500) with rat brain tissue lysate 4000ug.
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Background
RasGRF1, a member of calmodulin-activated guanine-nucleotide exchange factors (GEFs), highly expressed at the synaptic junctions of the central nervous system (CNS). It is similar to the Saccharomyces cerevisiae CDC25. RASGRF1 serves as an in vivo activator for H-RAS and members of the R-RAS and RAC subfamilies. Several studies have shown that RasGRF1 also binds to microtubules and participate in the regulation of neurite outgrowth. RasGRF1 has been implicated in neuronal excitability. The studies of the similar gene in mouse suggested that the Ras-GEF activity of this protein in brain can be activated by Ca2+ influx, muscarinic receptors, and G protein beta-gamma subunit. Mouse studies also indicated that the Ras-GEF signaling pathway mediated by this protein may be important for long-term memory.
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References
- Liao W, Jordaan G, Coriaty N, Sharma S. Amplification of B cell receptor-Erk signaling by Rasgrf-1 overexpression in chronic lymphocytic leukemia. Leukemia & lymphoma. 55(12):2907-16. 2014.
- Huang W, Liu J, Feng X. DLC-1 induces mitochondrial apoptosis and epithelial mesenchymal transition arrest in nasopharyngeal carcinoma by targeting EGFR/Akt/NF-κB pathway. Medical oncology (Northwood, London, England). 32(4):115. 2015.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"