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Product Name
RASGAP antibody
- Documents
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Description
RASGAP Rabbit Polyclonal antibody. Positive IHC detected in human testis tissue, human breast cancer tissue. Positive IP detected in mouse testis tissue. Positive WB detected in mouse testis tissue. Observed molecular weight by Western-blot: 120 kDa, 95 kDa
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Tested applications
ELISA, WB, IHC, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
CM AVM antibody; CMAVM antibody; DKFZp434N071 antibody; GAP antibody; GTPase activating protein antibody; p120GAP antibody; p120RASGAP antibody; PKWS antibody; Ras p21 protein activator antibody; RASA antibody; RASA1 antibody; RASGAP antibody
- Immunogen
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of RASGAP recombinant protein (Accession Number: NM_002890). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IP: 1:200-1:2000
IHC: 1:20-1:200
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Validations
mouse testis tissue were subjected to SDS PAGE followed by western blot with Catalog No:114474(RASA1 antibody) at dilution of 1:400
IP Result of anti-RASA1 (IP:Catalog No:114474, 4ug; Detection:Catalog No:114474 1:500) with mouse testis tissue lysate 4000ug.
Immunohistochemistry of paraffin-embedded human testis tissue slide using Catalog No:114474(RASA1 Antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemistry of paraffin-embedded human testis tissue slide using Catalog No:114474(RASA1 Antibody) at dilution of 1:50 (under 40x lens)
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Background
RASA1 represents RAS p21 protein activator (GTPase activating protein) 1
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References
- Wang J, Ruan K. miR-335 is involved in the rat epididymal development by targeting the mRNA of RASA1. Biochemical and biophysical research communications. 402(2):222-7. 2010.
- Ye X, Zhang Y, He B, Meng Y, Li Y, Gao Y. Quantitative proteomic analysis identifies new effectors of FOXM1 involved in breast cancer cell migration. International journal of clinical and experimental pathology. 8(12):15836-44. 2015.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"