RAG2 antibody

RAG2 antibody

• DataSheet
• Material Safety Data Sheets (MSDS)

RAG2 Rabbit Polyclonal antibody. Positive WB detected in A375 cells, mouse thymus tissue. Positive IP detected in A375 cells. Positive IHC detected in human lymphoma tissue. Positive IF detected in Hela cells. Positive FC detected in HeLa cells. Observed molecular weight by Western-blot: 57-62 kDa

ELISA, WB, IHC, IF, FC, IP

Human, Mouse; other species not tested.

RAG 2 antibody; RAG2 antibody

Rabbit IgG

This antibody was obtained by immunization of RAG2 recombinant protein (Accession Number: NM_001243785). Purification method: Antigen affinity purified.

Polyclonal

PBS with 0.1% sodium azide and 50% glycerol pH 7.3.

Store at -20℃. DO NOT ALIQUOT

Recombination activating gene 2(RAG2) is core part of the RAG complex(RAG1 and RAG2), which mediates the DNA cleavage phase during V(D)J recombination. The RAG complex also plays a role in pre-B cell allelic exclusion, a process leading to expression of a single immunoglobulin heavy chain allele to enforce clonality and monospecific recognition by the B-cell antigen receptor (BCR) expressed on individual B-lymphocytes. The introduction of DNA breaks by the RAG complex on one immunoglobulin allele induces ATM-dependent repositioning of the other allele to pericentromeric heterochromatin, preventing accessibility to the RAG complex and recombination of the second allele. In the RAG complex, RAG2 is not the catalytic component but is required for all known catalytic activities mediated by RAG1. It probably acts as a sensor of chromatin state that recruits the RAG complex to H3K4me3

• Hauser J, Grundström C, Grundström T. Allelic exclusion of IgH through inhibition of E2A in a VDJ recombination complex. Journal of immunology (Baltimore, Md. : 1950). 192(5):2460-70. 2014.