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Product Name
RAC2 antibody
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Description
RAC2 Rabbit Polyclonal antibody. Positive WB detected in HL-60 cells. Positive IP detected in HL-60 cells. Observed molecular weight by Western-blot: 21 kDa
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Tested applications
ELISA, WB, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
EN 7 antibody; HSPC022 antibody; p21 Rac2 antibody; RAC2 antibody; Small G protein antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of RAC2 recombinant protein (Accession Number: NM_002872). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IP: 1:200-1:1000
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Validations
HL-60 cells were subjected to SDS PAGE followed by western blot with Catalog No:114506(RAC2 Antibody) at dilution of 1:600
IP Result of anti-RAC2 (IP:Catalog No:114506, 3ug; Detection:Catalog No:114506 1:300) with HL-60 cells lysate 4000ug.
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Background
RAC2, also named as GX, belongs to the small GTPase superfamily and Rho family. RAC2 is a plasma membrane-associated small GTPase which cycles between an active GTP-bound and inactive GDP-bound state. In active state binds to a variety of effector proteins to regulate cellular responses, such as secretory processes, phagocytose of apoptotic cells and epithelial cell polarization. RAC2 augments the production of reactive oxygen species (ROS) by NADPH oxidase. Defects in RAC2 are the cause of neutrophil immunodeficiency syndrome.
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References
- Meacham CE, Ho EE, Dubrovsky E, Gertler FB, Hemann MT. In vivo RNAi screening identifies regulators of actin dynamics as key determinants of lymphoma progression. Nature genetics. 41(10):1133-7. 2009.
- Zeng Z, Bo H, Gong Z. AFAP1-AS1, a long noncoding RNA upregulated in lung cancer and promotes invasion and metastasis. Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine. 2015.
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