Product Name

RAC2 antibody

Documents

Description

RAC2 Mouse Monoclonal antibody. Positive IHC detected in human spleen tissue, human tonsillitis tissue. Positive WB detected in HL60 cells, Jurkat cells. Observed molecular weight by Western-blot: 21 kDa

Tested applications

ELISA, WB, IHC

Species reactivity

Human, Mouse; other species not tested.

Alternative names

EN 7 antibody; HSPC022 antibody; p21 Rac2 antibody; RAC2 antibody; Small G protein antibody

Isotype

Mouse IgG1

Preparation

This antibody was obtained by immunization of RAC2 recombinant protein (Accession Number: NM_002872). Purification method: Protein G purified.

Clonality

Monoclonal

Formulation

PBS with 0.1% sodium azide and 50% glycerol pH 7.3.

Storage instructions

Store at -20℃. DO NOT ALIQUOT

Applications

Recommended Dilution:

WB: 1:500-1:5000

IHC: 1:20-1:200

Validations

HL-60 cells were subjected to SDS PAGE followed by western blot with Catalog No:107495(RAC2 antibody) at dilution of 1:500

Immunohistochemistry of paraffin-embedded human spleen slide using Catalog No:107495(RAC2 Antibody) at dilution of 1:50

Background

RAC2, also named as GX, belongs to the small GTPase superfamily and Rho family. RAC2 is a plasma membrane-associated small GTPase which cycles between an active GTP-bound and inactive GDP-bound state. In active state binds to a variety of effector proteins to regulate cellular responses, such as secretory processes, phagocytose of apoptotic cells and epithelial cell polarization. RAC2 augments the production of reactive oxygen species (ROS) by NADPH oxidase. Defects in RAC2 are the cause of neutrophil immunodeficiency syndrome.

References

Morrison AR, Yarovinsky TO, Young BD. Chemokine-coupled β2 integrin-induced macrophage Rac2-Myosin IIA interaction regulates VEGF-A mRNA stability and arteriogenesis. The Journal of experimental medicine. 211(10):1957-68. 2014.
Bo H, Gong Z, Zhang W. Upregulated long non-coding RNA AFAP1-AS1 expression is associated with progression and poor prognosis of nasopharyngeal carcinoma. Oncotarget. 6(24):20404-18. 2015.
Wang ZJ, Song L, Guo LC, Yin M, Sun YN. Induction of differentiation by panaxydol in human hepatocarcinoma SMMC-7721 cells via cAMP and MAP kinase dependent mechanism. Yakugaku zasshi : Journal of the Pharmaceutical Society of Japan. 131(6):993-1000. 2011.
Cavnar PJ, Mogen K, Berthier E, Beebe DJ, Huttenlocher A. The actin regulatory protein HS1 interacts with Arp2/3 and mediates efficient neutrophil chemotaxis. The Journal of biological chemistry. 287(30):25466-77. 2012.

RAC2 antibody

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