Mouse AKT3 (aa 106-479) recombinant protein

Mouse AKT3 (aa 106-479) recombinant protein

• DataSheet
• Material Safety Data Sheets (MSDS)

AKT3 is one of 3 closely related serine/threonine-protein kinases (AKT1, AKT2 and AKT3) called the AKT kinase, and which regulate many processes including metabolism, proliferation, cell survival, growth and angiogenesis. This is mediated through serine and/or threonine phosphorylation of a range of downstream substrates. Over 100 substrate candidates have been reported so far, but for most of them, no isoform specificity has been reported. AKT3 is the least studied AKT isoform. It plays an important role in brain development and is crucial for the viability of malignant glioma cells. AKT3 isoform may also be the key molecule in up-regulation and down-regulation of MMP13 via IL13. Required for the coordination of mitochondrial biogenesis with growth factor-induced increases in cellular energy demands. Down-regulation by RNA interference reduces the expression of the phosphorylated form of BAD, resulting in the induction of caspase-dependent apoptosis.

RAC-gamma serine/threonine-protein kinase

PRKBG; PKBG; NMF350; D930002M15RIK; MPPH; AKT3; STK-2; RAC-GAMMA; I851531; AI851531; PKB-GAMMA; RAC-PK-GAMMA; MPPH2; DKFZP434N0250

Q9WUA6

Akt3

Baculovirus-Insect Cells

A DNA sequence encoding the AKT3 (Q9WUA6-1) (Ala106-Glu479) was expressed and purified.

Mouse

The recombinant mouse AKT3 consists of 376 amino acids and predicts a molecular mass of 43.4 KDa. It migrates as an approximately 46 KDa band in SDS-PAGE under reducing conditions.

> 95 % as determined by SDS-PAGE

The specific activity was determined to be 20 nmol/min/mg using synthetic GSK3-derived peptide (R11-SGRARTSSFAEPGGK) as substrate.