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Product Name
RAB3C antibody
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Description
RAB3C Rabbit Polyclonal antibody. Positive IHC detected in human ovary tumor tissue. Positive IP detected in mouse brain tissue. Positive WB detected in mouse lung tissue, human brain tissue, mouse brain tissue. Observed molecular weight by Western-blot: 28 kDa
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Tested applications
ELISA, WB, IHC, IP
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Species reactivity
Human, Mouse; other species not tested.
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Alternative names
RAB3C antibody; Ras related protein Rab 3C antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of RAB3C recombinant protein (Accession Number: NM_138453). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IP: 1:200-1:1000
IHC: 1:20-1:200
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Validations
mouse lung tissue were subjected to SDS PAGE followed by western blot with Catalog No:114442(RAB3C antibody) at dilution of 1:1000
IP Result of anti-RAB3C (IP:Catalog No:114442, 4ug; Detection:Catalog No:114442 1:300) with mouse brain tissue lysate 4000ug.
Immunohistochemical of paraffin-embedded human ovary tumor using Catalog No:114442(RAB3C antibody) at dilution of 1:100 (under 10x lens)
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Background
Rab proteins are small molecular weight GTPases that control vesicular traffic in eukaryotic cells. A subset of Rab proteins, the Rab3 proteins (Rab3A, Rab3B, Rab3C, and Rab3D) are thought to play an important role in regulated exocytosis of vesicles. Although Rab3 proteins are highly homologous, displaying 77-85% amino acid identity with greatest variance evident within the N- and C-terminal regions, their subcellular targets and functional roles remain somewhat distinct between them. RT-PCR analysis indicated that human Rab3C was expressed in the human brain, placenta, and lung.
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References
- Zou L, Zhou J, Zhang J. The GTPase Rab3b/3c-positive recycling vesicles are involved in cross-presentation in dendritic cells. Proceedings of the National Academy of Sciences of the United States of America. 106(37):15801-6. 2009.
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