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Product Name
RAB3A-specific antibody
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Description
RAB3A-specific Rabbit Polyclonal antibody. Positive WB detected in mouse brain tissue, rat brain tissue. Positive IF detected in HeLa cells. Positive IHC detected in human pancreas tissue. Observed molecular weight by Western-blot: 25 kDa
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Tested applications
ELISA, WB, IF, IHC
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
RAB3A antibody; Ras related protein Rab 3A antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of Peptide (Accession Number: NM_002866). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IHC: 1:20-1:200
IF: 1:10-1:100
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Validations
Immunohistochemistry of paraffin-embedded human pancreas tissue slide using Catalog No:114440(RAB3A-specific Antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemistry of paraffin-embedded human pancreas tissue slide using Catalog No:114440(RAB3A-specific Antibody) at dilution of 1:50 (under 40x lens)
Immunofluorescent analysis of HeLa cells using Catalog No:114440(RAB3A-specific Antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG
mouse brain tissue were subjected to SDS PAGE followed by western blot with Catalog No:114440(RAB3A-specific antibody) at dilution of 1:1000
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Background
The Rab3 subfamily includes Rab3A, Rab3B, Rab3C, and Rab3D, involving in the exocytosis of neurotransmitters and hormones. Rab 3A is found to be abundant in presynaptic nerve terminals and is also found to be crucial in acrosomal exocytosis in human spermatozoa. This antibody is specific to Rab3A.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"