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Product Name
Rab23 antibody
- Documents
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Description
Rab23 Rabbit Polyclonal antibody. Positive IHC detected in human liver cancer tissue. Positive IP detected in mouse brain tissue. Positive WB detected in mouse brain tissue, human brain tissue, rat brain tissue. Observed molecular weight by Western-blot: 27 kDa
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Tested applications
ELISA, WB, IP, IHC
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
DKFZp781H0695 antibody; HSPC137 antibody; RAB23 antibody; Ras related protein Rab 23 antibody
- Immunogen
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of Rab23 recombinant protein (Accession Number: NM_001278666). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:500-1:5000
IHC: 1:50-1:500
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Validations
mouse brain tissue were subjected to SDS PAGE followed by western blot with Catalog No:114422(RAB23 antibody) at dilution of 1:1000
Immunohistochemistry of paraffin-embedded human liver cancer tissue slide using Catalog No:114422(RAB23 Antibody) at dilution of 1:200 (under 10x lens).
Immunohistochemistry of paraffin-embedded human liver cancer tissue slide using Catalog No:114422(RAB23 Antibody) at dilution of 1:200 (under 40x lens).
IP Result of anti-RAB23 (IP:Catalog No:114422, 3ug; Detection:Catalog No:114422 1:1000) with mouse brain tissue lysate 7000ug.
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Background
Rab23 is a novel member of large Rab small GTPase family. It plays important roles during embryonic development. The brain-enriched Rab23 is the only Rab protein that is known to have a distinct function during central nervous system development, playing an essential role as a negative regulator of the Sonic Hedgehog (Shh) pathway. RAB23 has recently been identified in mesangial cells (MCs), and could serve as a biomarker that indicates the severity of FSGS ( focal segmental glomerulosclerosis). Mutations in RAB23 may result in ACPS2 ( acrocephalopolysyndactyly type 2).
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References
- Boehlke C, Bashkurov M, Buescher A. Differential role of Rab proteins in ciliary trafficking: Rab23 regulates smoothened levels. Journal of cell science. 123(Pt 9):1460-7. 2010.
- Jian Q, Miao Y, Tang L. Rab23 promotes squamous cell carcinoma cell migration and invasion via integrin β1/Rac1 pathway. Oncotarget. 2015.
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