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Product Name
RAB10 antibody
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Description
RAB10 Rabbit Polyclonal antibody. Positive WB detected in human brain tissue, mouse brain tissue, mouse skeletal muscle tissue, rat brain tissue. Positive IHC detected in human prostate cancer tissue, human colon cancer tissue. Observed molecular weight by Western-blot: 29 kDa
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Tested applications
ELISA, WB, IHC
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
RAB10 antibody; Ras related protein Rab 10 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of RAB10 recombinant protein (Accession Number: NM_016131). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IHC: 1:20-1:200
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Validations
human brain tissue were subjected to SDS PAGE followed by western blot with Catalog No:114404(RAB10 antibody) at dilution of 1:400
Immunohistochemical of paraffin-embedded human prostate cancer using Catalog No:114404(RAB10 antibody) at dilution of 1:100 (under 10x lens)
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Background
RAB10 belongs to the RAS superfamily of small GTPases. RAB10 localizes to a system of tubules and vesicles, and it may be involved in vesicular trafficking and neurotransmitter release. It is also associated with Glut4 translocation in adipocytes.
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References
- Zhang Y, Ye Y, Shen D. Identification of transgelin-2 as a biomarker of colorectal cancer by laser capture microdissection and quantitative proteome analysis. Cancer science. 101(2):523-9. 2010.
- Wang D, Lou J, Ouyang C. Ras-related protein Rab10 facilitates TLR4 signaling by promoting replenishment of TLR4 onto the plasma membrane. Proceedings of the National Academy of Sciences of the United States of America. 107(31):13806-11. 2010.
- Lacruz RS, Brookes SJ, Wen X. Adaptor protein complex 2-mediated, clathrin-dependent endocytosis, and related gene activities, are a prominent feature during maturation stage amelogenesis. Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research. 28(3):672-87. 2013.
- Liu Y, Xu XH, Chen Q. Myosin Vb controls biogenesis of post-Golgi Rab10 carriers during axon development. Nature communications. 4:2005. 2013.
- Pavlos NJ, Grønborg M, Riedel D. Quantitative analysis of synaptic vesicle Rabs uncovers distinct yet overlapping roles for Rab3a and Rab27b in Ca2+-triggered exocytosis. The Journal of neuroscience : the official journal of the Society for Neuroscience. 30(40):13441-53. 2010.
- Deng CY, Lei WL, Xu XH, Ju XC, Liu Y, Luo ZG. JIP1 mediates anterograde transport of Rab10 cargos during neuronal polarization. The Journal of neuroscience : the official journal of the Society for Neuroscience. 34(5):1710-23. 2014.
- Xu XH, Deng CY, Liu Y. MARCKS regulates membrane targeting of Rab10 vesicles to promote axon development. Cell research. 24(5):576-94. 2014.
- Wu KY, He M, Hou QQ. Semaphorin 3A activates the guanosine triphosphatase Rab5 to promote growth cone collapse and organize callosal axon projections. Science signaling. 7(340):ra81. 2014.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"