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Product Name
PYGO2 antibody
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Description
PYGO2 Rabbit Polyclonal antibody. Positive IHC detected in human breast cancer tissue. Positive WB detected in MDA-MB-453s cells, C6 cells, HeLa cells. Observed molecular weight by Western-blot: 50 kDa
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Tested applications
ELISA, IHC, WB
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
1190004M21Rik antibody; FLJ33226 antibody; PYGO2 antibody; Pygopus homolog 2 antibody; pygopus homolog 2 (Drosophila) antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of PYGO2 recombinant protein (Accession Number: NM_138300). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IHC: 1:20-1:200
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Validations
Immunohistochemical of paraffin-embedded human breast cancer using Catalog No:114362(PYGO2 antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemical of paraffin-embedded human breast cancer using Catalog No:114362(PYGO2 antibody) at dilution of 1:50 (under 40x lens)
MDA-MB-453s cells were subjected to SDS PAGE followed by western blot with Catalog No:114362(PYGO2 Antibody) at dilution of 1:600
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Background
PYGO2, also named as Pygopus homolog 2, is a 406 animo acid protein, which contains 1 PHD-type zinc finger. PYGO2 is involved in signal transduction through the Wnt pathway and Binds to BCL9 via the PHD-type zinc finger motif, and thereby becomes part of the nuclear beta-catenin/TCF complex. PYGO2 is in a high proportion of breast and epithelial ovarian malignant tumors and is required for the growth of several cell lines derived from these carcinomas.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"