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Product Name
PYCR2 antibody
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Description
PYCR2 Rabbit Polyclonal antibody. Positive IHC detected in human ovary tissue, human kidney tissue, human lung tissue, human placenta tissue, human testis tissue. Positive IF detected in Hela cells. Positive IP detected in Jurkat cells. Positive WB detected in Jurkat cells, HeLa cells, L02 cells. Observed molecular weight by Western-blot: 34 kDa
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Tested applications
ELISA, WB, IHC, IF, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
FLJ54750 antibody; P5C reductase 2 antibody; P5CR 2 antibody; P5CR2 antibody; PYCR2 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of PYCR2 recombinant protein (Accession Number: NM_013328). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IP: 1:200-1:2000
IHC: 1:20-1:200
IF: 1:10-1:100
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Validations
Jurkat cells were subjected to SDS PAGE followed by western blot with Catalog No:114355(PYCR2 antibody) at dilution of 1:800
Immunohistochemical of paraffin-embedded human ovary using Catalog No:114355(PYCR2 antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemical of paraffin-embedded human ovary using Catalog No:114355(PYCR2 antibody) at dilution of 1:50 (under 40x lens)
Immunofluorescent analysis of Hela cells, using PYCR2 antibody Catalog No:114355 at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
IP Result of anti-PYCR2 (IP:Catalog No:114355, 4ug; Detection:Catalog No:114355 1:500) with Jurkat cells lysate 2400ug.
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Background
PYCR2 represents pyrroline-5-carboxylate reductase family, member 2
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References
- Liu W, Hancock CN, Fischer JW, Harman M, Phang JM. Proline biosynthesis augments tumor cell growth and aerobic glycolysis: involvement of pyridine nucleotides. Scientific reports. 5:17206. 2015.
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