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Product Name
PTPN23 antibody
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Description
PTPN23 Rabbit Polyclonal antibody. Positive IHC detected in human colon cancer tissue. Positive IP detected in HeLa cells. Positive WB detected in A375 cells, HeLa cells. Observed molecular weight by Western-blot: 179 kDa
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Tested applications
ELISA, WB, IHC, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
DKFZp564F0923 antibody; HD PTP antibody; HDPTP antibody; KIAA1471 antibody; PTP TD14 antibody; PTPN23 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of PTPN23 recombinant protein (Accession Number: BC004881). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:200-1:2000
IHC: 1:20-1:200
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Validations
A375 cells were subjected to SDS PAGE followed by western blot with Catalog No:114320(PTPN23 antibody) at dilution of 1:500
Immunohistochemical of paraffin-embedded human colon cancer using Catalog No:114320(PTPN23 antibody) at dilution of 1:100 (under 10x lens)
IP Result of anti-PTPN23 (IP:Catalog No:114320, 5ug; Detection:Catalog No:114320 1:500) with HeLa cells lysate 1000ug.
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Background
PTPN23(Tyrosine-protein phosphatase non-receptor type 23) is also named as HD-PTP,PTP-TD14 and KIAA1471.It encodes a 185-kDa protein which is expressed in human endothelial fromthe umbilical cord and in human Kaposi-spindle cells(PMID:16408268).PTPN23 is a non-transmembrane protein tyrosine phosphatase which contributes to the signal transduction pathways involved in the regulation of cell migration and invasion(PMID:22510412).
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References
- Ali N, Zhang L, Taylor S, Mironov A, Urbé S, Woodman P. Recruitment of UBPY and ESCRT exchange drive HD-PTP-dependent sorting of EGFR to the MVB. Current biology : CB. 23(6):453-61. 2013.
- Chen DY, Li MY, Wu SY. The Bro1-domain-containing protein Myopic/HDPTP coordinates with Rab4 to regulate cell adhesion and migration. Journal of cell science. 125(Pt 20):4841-52. 2012.
- Stefani F, Zhang L, Taylor S. UBAP1 is a component of an endosome-specific ESCRT-I complex that is essential for MVB sorting. Current biology : CB. 21(14):1245-50. 2011.
- Tanaka K, Kondo K, Kitajima K, Muraoka M, Nozawa A, Hara T. Tumor-suppressive function of protein-tyrosine phosphatase non-receptor type 23 in testicular germ cell tumors is lost upon overexpression of miR142-3p microRNA. The Journal of biological chemistry. 288(33):23990-9. 2013.
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