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Product Name
PTGES3 antibody
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Description
PTGES3 Rabbit Polyclonal antibody. Positive IF detected in Hela cells. Positive FC detected in HeLa cells. Positive IP detected in mouse heart tissue. Positive WB detected in rat brain tissue, mouse brain tissue, mouse heart tissue, rat heart tissue. Observed molecular weight by Western-blot: 21-23kd
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Tested applications
ELISA, IF, IP, WB, FC
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
cPGES antibody; Hsp90 co chaperone antibody; P23 antibody; Prostaglandin E synthase 3 antibody; PTGES3 antibody; TEBP antibody; Telomerase binding protein p23 antibody
- Immunogen
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of PTGES3 recombinant protein (Accession Number: NM_006601). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:200-1:1000
IF: 1:10-1:100
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Validations
rat brain tissue were subjected to SDS PAGE followed by western blot with Catalog No:114271(PTGES3 Antibody) at dilution of 1:1000
Immunofluorescent analysis of Hela cells, using PTGES3 antibody Catalog No:114271 at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
IP Result of anti-PTGES3 (IP:Catalog No:114271, 3ug; Detection:Catalog No:114271 1:300) with mouse heart tissue lysate 4000ug.
1X10^6 HeLa cells were stained with 0.2ug PTGES3 antibody (Catalog No:114271, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.
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Background
P23, encoded by the gene PTGES3, plays a key role in glucocorticoid signaling and was increased at the mRNA level in the DLPFC in individuals with schizophrenia (PMID: 24345775). In the GR heterocomplex in vitro, p23 is an obligatory co-factor19 and is the limiting component of the complex44, functioning to stabilize the interaction of the complex with GR in the cytoplasm. Paradoxically, p23 also acts in the nucleus to inhibit GR-mediated gene transcription and disassemble GR transcriptional machinery in the nucleus (PMID: 12077419). In vivo, p23 is critical for appropriate glucocorticoid responsiveness (PMID: 17000766).
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