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Product Name
PTGES2 antibody
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Description
PTGES2 Rabbit Polyclonal antibody. Positive IF detected in Hela cells, HepG2 cells. Positive IHC detected in human breast cancer tissue, human colon cancer tissue, human heart tissue. Positive IP detected in mouse heart tissue. Positive WB detected in HeLa cells, HEK-293 cells, HepG2 cells, human stomach tissue, mouse heart tissue. Observed molecular weight by Western-blot: 30-35 kDa,45 kDa
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Tested applications
ELISA, IHC, IF, WB, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
C9orf15 antibody; FLJ14038 antibody; GBF1 antibody; mPGES 2 antibody; PGES2 antibody; prostaglandin E synthase 2 antibody; PTGES2 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of PTGES2 recombinant protein (Accession Number: NM_025072). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IP: 1:200-1:2000
IHC: 1:20-1:200
IF: 1:10-1:100
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Validations
Immunohistochemical of paraffin-embedded human breast cancer using Catalog No:114270(PTGES2 antibody) at dilution of 1:50 (under 40x lens)
Immunofluorescent analysis of Hela cells, using PTGES2 antibody Catalog No:114270 at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:114270(PTGES2 Antibody) at dilution of 1:600
IP Result of anti-PTGES2 (IP:Catalog No:114270, 4ug; Detection:Catalog No:114270 1:500) with mouse heart tissue lysate 4000ug.
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Background
PTGES2(Prostaglandin E synthase 2) catalyzes the conversion of unstable intermediate of prostaglandin E2 H2 (PGH2) into the more stable prostaglandin E2 (PGE2) form.Its predictedsize is 42 KDa,but it was detected one band of 35KDa in the prostate and two bands of 30KDa,50KDa in liver by western blot(PMID:17566096). PTGES2 protein is a homotetramer, whereas two PTGES2 molecules each form a strongly interacting dimer(PMID:19268562).
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References
- Kim JD, Ahn BM, Joo BS, Kwon JY, Chung HJ, Yu SB. Effect of propofol on prostaglandin E2 production and prostaglandin synthase-2 and cyclooxygenase-2 expressions in amniotic membrane cells. Journal of anesthesia. 28(6):911-8. 2014.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"