PTGES2 antibody

Cat.#: 114270

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Product Information

  • Product Name
    PTGES2 antibody
  • Documents
  • Description
    PTGES2 Rabbit Polyclonal antibody. Positive IF detected in Hela cells, HepG2 cells. Positive IHC detected in human breast cancer tissue, human colon cancer tissue, human heart tissue. Positive IP detected in mouse heart tissue. Positive WB detected in HeLa cells, HEK-293 cells, HepG2 cells, human stomach tissue, mouse heart tissue. Observed molecular weight by Western-blot: 30-35 kDa,45 kDa
  • Tested applications
    ELISA, IHC, IF, WB, IP
  • Species reactivity
    Human,Mouse,Rat; other species not tested.
  • Alternative names
    C9orf15 antibody; FLJ14038 antibody; GBF1 antibody; mPGES 2 antibody; PGES2 antibody; prostaglandin E synthase 2 antibody; PTGES2 antibody
  • Isotype
    Rabbit IgG
  • Preparation
    This antibody was obtained by immunization of PTGES2 recombinant protein (Accession Number: NM_025072). Purification method: Antigen affinity purified.
  • Clonality
    Polyclonal
  • Formulation
    PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
  • Storage instructions
    Store at -20℃. DO NOT ALIQUOT
  • Applications

    Recommended Dilution:

    WB: 1:200-1:2000

    IP: 1:200-1:2000

    IHC: 1:20-1:200

    IF: 1:10-1:100

  • Validations

    Immunohistochemical of paraffin-embedded human breast cancer using Catalog No:114270(PTGES2 antibody) at dilution of 1:50 (under 40x lens)

    Immunohistochemical of paraffin-embedded human breast cancer using Catalog No:114270(PTGES2 antibody) at dilution of 1:50 (under 40x lens)

    Immunofluorescent analysis of Hela cells, using PTGES2 antibody Catalog No:114270 at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

    Immunofluorescent analysis of Hela cells, using PTGES2 antibody Catalog No:114270 at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

    HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:114270(PTGES2 Antibody) at dilution of 1:600

    HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:114270(PTGES2 Antibody) at dilution of 1:600

    IP Result of anti-PTGES2 (IP:Catalog No:114270, 4ug; Detection:Catalog No:114270 1:500) with mouse heart tissue lysate 4000ug.

    IP Result of anti-PTGES2 (IP:Catalog No:114270, 4ug; Detection:Catalog No:114270 1:500) with mouse heart tissue lysate 4000ug.

  • Background
    PTGES2(Prostaglandin E synthase 2) catalyzes the conversion of unstable intermediate of prostaglandin E2 H2 (PGH2) into the more stable prostaglandin E2 (PGE2) form.Its predictedsize is 42 KDa,but it was detected one band of 35KDa in the prostate and two bands of 30KDa,50KDa in liver by western blot(PMID:17566096). PTGES2 protein is a homotetramer, whereas two PTGES2 molecules each form a strongly interacting dimer(PMID:19268562).
  • References
    • Kim JD, Ahn BM, Joo BS, Kwon JY, Chung HJ, Yu SB. Effect of propofol on prostaglandin E2 production and prostaglandin synthase-2 and cyclooxygenase-2 expressions in amniotic membrane cells. Journal of anesthesia. 28(6):911-8. 2014.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"