-
Product Name
PSME3 antibody
- Documents
-
Description
PSME3 Rabbit Polyclonal antibody. Positive WB detected in human colon tissue, mouse spleen tissue. Positive IP detected in COLO 320 cells. Positive IHC detected in human colon cancer tissue, human brain tissue, human heart tissue, human kidney tissue, human lung tissue, human ovary tissue, human placenta tissue, human skin tissue, human spleen tissue, human testis tissue. Positive IF detected in HepG2 cells. Observed molecular weight by Western-blot: 30 kDa
-
Tested applications
ELISA, WB, IHC, IF, IP
-
Species reactivity
Human,Mouse,Rat; other species not tested.
-
Alternative names
Ki nuclear autoantigen antibody; PA28 gamma antibody; PA28G antibody; PA28gamma antibody; PSME3 antibody; REG GAMMA antibody
-
Isotype
Rabbit IgG
-
Preparation
This antibody was obtained by immunization of PSME3 recombinant protein (Accession Number: NM_005789). Purification method: Antigen affinity purified.
-
Clonality
Polyclonal
-
Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
-
Storage instructions
Store at -20℃. DO NOT ALIQUOT
-
Applications
Recommended Dilution:
WB: 1:200-1:2000
IP: 1:200-1:2000
IHC: 1:20-1:200
IF: 1:10-1:100
-
Validations
human colon tissue were subjected to SDS PAGE followed by western blot with Catalog No:114248(PSME3 antibody) at dilution of 1:500
Immunohistochemical of paraffin-embedded human colon cancer using Catalog No:114248(PSME3 antibody) at dilution of 1:50 (under 40x lens)
Immunofluorescent analysis of HepG2 cells, using PSME3 antibody Catalog No:114248 at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
IP Result of anti-PSME3 (IP:Catalog No:114248, 3ug; Detection:Catalog No:114248 1:500) with COLO 320 cells lysate 2000ug.
-
Background
PSME3 gene encodes proteasome activator complex subunit 3, which is also called REG-gamma or PA28-gamma. REG-gamma activates the trypsin-like catalytic subunit of the proteasome thus is a proteasome regulator. MDM2-TP53 interaction which promotes ubiquitination- and MDM2-dependent proteasomal degradation of TP53, may be promoted by REG-gamma resulting in inhibited apoptosis after DNA damage. REG-gamma may also be involved in cell cycle regulation.
-
References
- Doueiri R, Anupam R, Kvaratskhelia M, Green KB, Lairmore MD, Green PL. Comparative host protein interactions with HTLV-1 p30 and HTLV-2 p28: insights into difference in pathobiology of human retroviruses. Retrovirology. 9:64. 2012.
Related Products / Services
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"