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Product Name
PRSS1 Polyclonal Antibody
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Description
Polyclonal antibody to PRSS1
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Tested applications
WB, IHC
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Species reactivity
Human, Mouse
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Alternative names
PRSS1 antibody; TRP1 antibody; TRY1 antibody; TRY4 antibody; TRYP1 antibody; trypsin-1 antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 24-247 of human PRSS1 (NP_002760.1).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
IHC 1:100 - 1:200 -
Validations
Western blot - PRSS1 Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using PRSS1 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.
Immunohistochemistry - PRSS1 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded human lung cancer using PRSS1 antibody at dilution of 1:100 (40x lens).
Immunohistochemistry - PRSS1 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded human prostate using PRSS1 antibody at dilution of 1:100 (40x lens).
Immunohistochemistry - PRSS1 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded mouse lung using PRSS1 antibody at dilution of 1:100 (40x lens).
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Background
Has activity against the synthetic substrates Boc-Phe-Ser-Arg-Mec, Boc-Leu-Thr-Arg-Mec, Boc-Gln-Ala-Arg-Mec and Boc-Val-Pro-Arg-Mec. The single-chain form is more active than the two-chain form against all of these substrates.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"