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Product Name
PRR5 antibody
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Description
PRR5 Rabbit Polyclonal antibody. Positive IHC detected in human placenta tissue, human brain tissue, human heart tissue, human kidney tissue, human lung tissue, human ovary tissue, human skin tissue, human spleen tissue, human testis tissue. Positive IF detected in HepG2 cells. Positive WB detected in human kidney tissue, COLO 320 cells, HEK-293 cells, HepG2 cells, human brain tissue, mouse brain tissue. Positive IP detected in mouse brain tissue. Observed molecular weight by Western-blot: 33 kDa,50 kDa
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Tested applications
ELISA, WB, IHC, IF, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
FLJ20185 antibody; FLJ20185k antibody; PP610 antibody; proline rich 5 (renal) antibody; Proline rich protein 5 antibody; Protein observed with Rictor 1 antibody; Protor 1 antibody; PROTOR1 antibody; PRR5 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of PRR5 recombinant protein (Accession Number: BC016921). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:200-1:1000
IHC: 1:20-1:200
IF: 1:10-1:100
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Validations
human kidney tissue were subjected to SDS PAGE followed by western blot with Catalog No:114276(PRR5 antibody) at dilution of 1:500
Immunohistochemical of paraffin-embedded human placenta using Catalog No:114276(PRR5 antibody) at dilution of 1:100 (under 10x lens)
Immunohistochemical of paraffin-embedded human placenta using Catalog No:114276(PRR5 antibody) at dilution of 1:100 (under 40x lens)
Immunofluorescent analysis of HepG2 cells, using PRR5 antibody Catalog No:114276 at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
IP Result of anti-PRR5 (IP:Catalog No:114276, 3ug; Detection:Catalog No:114276 1:300) with mouse brain tissue lysate 4000ug.
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Background
PRR5 represents proline rich 5 (renal)
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