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Product Name
PRPF3 antibody
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Description
PRPF3 Rabbit Polyclonal antibody. Positive WB detected in Y79 cells, HepG2 cells, L02 cells, mouse brain tissue, mouse liver tissue. Positive IP detected in mouse liver tissue. Observed molecular weight by Western-blot: 90 kDa or 78 kDa
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Tested applications
ELISA, WB, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
HPRP3 antibody; HPRP3P antibody; Pre mRNA splicing factor 3 antibody; PRP3 antibody; Prp3p antibody; PRPF3 antibody; RP18 antibody; U4/U6 snRNP 90 kDa protein antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of PRPF3 recombinant protein (Accession Number: XM_047433995). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:200-1:2000
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Validations
Y79 cells were subjected to SDS PAGE followed by western blot with Catalog No:114228(PRPF3 antibody) at dilution of 1:1000
IP Result of anti-PRPF3 (IP:Catalog No:114228, 5ug; Detection:Catalog No:114228 1:500) with mouse liver tissue lysate 4000ug.
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Background
The pre-mRNA splicing occurs in spliceosomes, which consist of four ribonucleoprotein (snRNP) particles (U1, U2, U5 and U4/U6) and more than fifty proteins. This U4/U6-associated splicing factor, Hprp3p, is a 77 kDa protein, which is homologous to the Saccharomyces cerevisiae splicing factor Prp3p and may play a role in spliceosome assembly. Western blot analysis of HeLa cell nuclear extracts detected PRP3 protein at an apparent molecular mass of about 90 kD(PMID: 9328476 ). The discrepancy between the molecular mass reported here and that predicted from the Hprp3p coding sequence can be attributed, at least partially, to the highly positive charge; its value of predicted isoelectric point (IP) is ∼9.99. This antibody is a rabbit polyclonal antibody raised against an internal region of human PRPF3.
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References
- Tanackovic G, Ransijn A, Thibault P. PRPF mutations are associated with generalized defects in spliceosome formation and pre-mRNA splicing in patients with retinitis pigmentosa. Human molecular genetics. 20(11):2116-30. 2011.
- Berg MG, Wan L, Younis I. A quantitative high-throughput in vitro splicing assay identifies inhibitors of spliceosome catalysis. Molecular and cellular biology. 32(7):1271-83. 2012.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"