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Product Name
Human TGM3/Transglutaminase 3 (His Tag) recombinant protein
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Description
Catalyzes the calcium-dependent formation of isopeptide cross-links between glutamine and lysine residues in various proteins, as well as the conjugation of polyamines to proteins. Involved in the formation of the cornified envelope (CE), a specialized component consisting of covalent cross-links of proteins beneath the plasma membrane of terminally differentiated keratinocytes. Catalyzes small proline-rich proteins (SPRR1 and SPRR2) and LOR cross-linking to form small interchain oligomers, which are further cross-linked by TGM1 onto the growing CE scaffold (By similarity). In hair follicles, involved in cross-linking structural proteins to hardening the inner root sheath.
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Protein name
Protein-glutamine gamma-glutamyltransferase E
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Protein short names
TGM3; TGE; MGC150256; TRANSGLUTAMINASE 3; TGASEE; MGC126250; MGC150257; RP23-263L18.1; AI893889; TGASE-3; MGC126249; TG(E)
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Uniprot ID
Q08188
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Gene Name
TGM3
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Source/Expression Host
Baculovirus-Insect Cells
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Expression Plasmid/cDNA
A DNA sequence encoding the human TGM3 (Q08188) (Ala 2-Glu 693) was expressed, with a polyhistidine tag at the N-terminus.
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Protein Species
Human
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Molecular weight
The recombinant human TGM3 consists of 710 amino acids and predicts a molecular mass of 78.8 kDa. It migrates as an approximately 70 kDa band in SDS-PAGE under reducing conditions.
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Purity
> 95 % as determined by SDS-PAGE
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Activity
Measured by its ability to cleave a synthetic peptide Benzyloxycarbonyl-Gln-Gly and NH2OH.
The specific activity is > 450 pmoles/min/μg. -
Validations
Human TGM3 / Transglutaminase 3 Protein (His Tag) SDS-PAGE
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