-
Product Name
TGM3 Polyclonal Antibody
- Documents
-
Description
Polyclonal antibody to TGM3
-
Tested applications
WB, IF
-
Species reactivity
Human, Mouse
-
Alternative names
TGM3 antibody; TGE antibody; UHS2 antibody; transglutaminase 3 antibody
-
Isotype
Rabbit IgG
-
Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-180 of human TGM3 (NP_003236.3).
-
Clonality
Polyclonal
-
Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
-
Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
-
Applications
WB 1:500 - 1:2000
IF 1:10 - 1:100 -
Validations
Western blot - TGM3 Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using TGM3 antibody at 1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit ._Exposure time: 50s.
Immunofluorescence - TGM3 Polyclonal Antibody
Immunofluorescence analysis of U2OS cells using TGM3 antibody . Blue: DAPI for nuclear staining.
-
Background
Catalyzes the calcium-dependent formation of isopeptide cross-links between glutamine and lysine residues in various proteins, as well as the conjugation of polyamines to proteins. Involved in the formation of the cornified envelope (CE), a specialized component consisting of covalent cross-links of proteins beneath the plasma membrane of terminally differentiated keratinocytes. Catalyzes small proline-rich proteins (SPRR1 and SPRR2) and LOR cross-linking to form small interchain oligomers, which are further cross-linked by TGM1 onto the growing CE scaffold (By similarity). In hair follicles, involved in cross-linking structural proteins to hardening the inner root sheath.
Related Products / Services
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"