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Product Name
Prohibitin antibody
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Description
Prohibitin Mouse Monoclonal antibody. Positive IF detected in MCF-7 cells, Hela cells. Positive IHC detected in human liver tissue, human heart tissue, human liver cancer tissue. Positive WB detected in Hela cells, human placenta tissue. Observed molecular weight by Western-blot: 30 kDa
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Tested applications
ELISA, WB, IHC, IF
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Species reactivity
Human, Mouse; other species not tested.
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Alternative names
PHB antibody; PHB1 antibody; prohibitin antibody
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Isotype
Mouse IgG1
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Preparation
This antibody was obtained by immunization of Prohibitin recombinant protein (Accession Number: NM_001281715). Purification method: Caprylic acid/ammonium sulfate precipitation.
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Clonality
Monoclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IHC: 1:20-1:200
IF: 1:10-1:100
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Validations
HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:107485(PHB antibody) at dilution of 1:500
Immunohistochemical of paraffin-embedded human liver using Catalog No:107485(PHB antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemical of paraffin-embedded human liver using Catalog No:107485(PHB antibody) at dilution of 1:50 (under 40x lens)
Immunofluorescent analysis of MCF-7 cells, using PHB antibody Catalog No: at 1:25 dilution and Rhodamine-labeled goat anti-mouse IgG (red).
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Background
Prohibitin, also known as PHB, a member of the Band-7 family of proteins, is widely distributed in bacteria, plants, yeast, protozoa and mammals and is important in cell proliferation, differentiation and apoptosis (PMID: 20840588 ). This protein localizes to the inner membrane of mitochondria, where it acts as a chaperone protein, and is also found in the nucleus, where it negatively regulates transcription (PMID: 18558096). Recent study confirmed that the expression and distribution of PHB, which is a nuclear matrix protein, affect the apoptosis of HaCaT cells and its co-localization with specific gene products connected with cell apoptosis (PMID: 24402549).
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"