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Product Name
Profilin 1 antibody
- Documents
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Description
Profilin 1 Rabbit Polyclonal antibody. Positive WB detected in human brain tissue, HeLa cells, Jurkat cells, NIH/3T3 cells. Positive IHC detected in human colon cancer tissue. Observed molecular weight by Western-blot: 15 kDa
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Tested applications
ELISA, WB, IHC
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
Epididymis tissue protein Li 184a antibody; PFN1 antibody; Profilin 1 antibody; Profilin I antibody
- Immunogen
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of Profilin 1 recombinant protein (Accession Number: NM_005022). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:1000-1:10000
IHC: 1:20-1:200
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Validations
human brain tissue were subjected to SDS PAGE followed by western blot with Catalog No:114214(PFN1 antibody) at dilution of 1:500
Immunohistochemical of paraffin-embedded human colon cancer using Catalog No:114214(PFN1 antibody) at dilution of 1:50 (under 10x lens)
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Background
Profilin-1 (PFN1) plays an important role in the control of actin dynamics, and could represent an important therapeutic target in several diseases. PFN1 is identified as a huntingtin aggregation inhibitor, and may serves as a tumor-suppressor. PFN1 is crucial for the conversion of monomeric (G)-actin to filamentous (F)-actin. Amyotrophic lateral sclerosis (ALS) is a late-onset neurodegenerative disorder resulting from motor neuron death. Cells expressing PFN1 mutants contain ubiquitinated, insoluble aggregates that in many cases contain the ALS-associated protein TDP-43. Recently, PFN1 is a potential biomarker for bladder cancer aggressiveness and may be of great clinical importance.
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References
- Yang D, Lü X, Hong Y, Xi T, Zhang D. The molecular mechanism for effects of TiN coating on NiTi alloy on endothelial cell function. Biomaterials. 35(24):6195-205. 2014.
- Zeng Z, Bo H, Gong Z. AFAP1-AS1, a long noncoding RNA upregulated in lung cancer and promotes invasion and metastasis. Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine. 2015.
- Bo H, Gong Z, Zhang W. Upregulated long non-coding RNA AFAP1-AS1 expression is associated with progression and poor prognosis of nasopharyngeal carcinoma. Oncotarget. 6(24):20404-18. 2015.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"