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Product Name
PRIM1 antibody
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Description
PRIM1 Rabbit Polyclonal antibody. Positive IHC detected in human lymphoma tissue. Positive IP detected in HeLa cells. Positive WB detected in K-562 cells, HeLa cells, Sp2/0 cells. Observed molecular weight by Western-blot: 50kd
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Tested applications
ELISA, WB, IHC, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
DNA primase 49 kDa subunit antibody; DNA primase small subunit antibody; p49 antibody; PRIM1 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of PRIM1 recombinant protein (Accession Number: NM_000946). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IP: 1:200-1:1000
IHC: 1:20-1:200
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Validations
K-562 cells were subjected to SDS PAGE followed by western blot with Catalog No:114192(PRIM1 antibody) at dilution of 1:500
Immunohistochemical of paraffin-embedded human lymphoma using Catalog No:114192(PRIM1 antibody) at dilution of 1:100 (under 10x lens)
IP Result of anti-PRIM1 (IP:Catalog No:114192, 4ug; Detection:Catalog No:114192 1:300) with HeLa cells lysate 2800ug.
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Background
DNA replication in human cells is initiated by a complex apparatus containing a DNA polymerase-alpha/primase complex. Primase synthesizes oligoribonucleotides that serve as primers for the initiation of DNA synthesis. It plays a role in both the initiation of DNA replication and the synthesis of Okazaki fragments for lagging strand synthesis. PRIM1 is a subnuit of this complex.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"