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Product Name
PRAP1 antibody
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Description
PRAP1 Rabbit Polyclonal antibody. Positive IHC detected in human liver tissue, human kidney tissue, mouse small intestine tissue. Positive WB detected in L02 cells, mouse pancreas tissue. Observed molecular weight by Western-blot: 16-20 kDa
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Tested applications
ELISA, WB, IHC
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Species reactivity
Human, Mouse; other species not tested.
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Alternative names
PRAP1 antibody; PRO1195 antibody; proline rich acidic protein 1 antibody; RP11 122K13.6 antibody; UPA antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of PRAP1 recombinant protein (Accession Number: BC029447). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IHC: 1:20-1:200
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Validations
L02 cells were subjected to SDS PAGE followed by western blot with Catalog No:114166(PRAP1 antibody) at dilution of 1:1000
Immunohistochemical of paraffin-embedded human liver using Catalog No:114166(PRAP1 antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemical of paraffin-embedded human liver using Catalog No:114166(PRAP1 antibody) at dilution of 1:50 (under 40x lens)
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Background
Proline rich acidic protein 1 (PRAP1) is also named as PRO1195 or UPA. It may play an important role in maintaining normal growth homeostasis in epithelial cells. Mitotic arrest deficient 1 (MAD1) is a key component in mitotic checkpoint signalling. Recent study identified PRAP1 as a novel MAD1 binding partner using yeast-two hybrid screening (PMID: 24374861). PRAP1 has four isoforms with MW 16-20 kDa and 10 kDa.
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