-
Product Name
PPP4R1 Polyclonal Antibody
- Documents
-
Description
Polyclonal antibody to PPP4R1
-
Tested applications
WB, IHC, IP
-
Species reactivity
Human, Mouse
-
Alternative names
PPP4R1 antibody; MEG1 antibody; PP4(Rmeg) antibody; PP4R1 antibody; protein phosphatase 4 regulatory subunit 1 antibody
-
Isotype
Rabbit IgG
-
Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 324-647 of human PPP4R1 (NP_001035847.1).
-
Clonality
Polyclonal
-
Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
-
Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
-
Applications
WB 1:500 - 1:2000
IHC 1:50 - 1:100
IP 1:50 - 1:100 -
Validations
Western blot - PPP4R1 Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using PPP4R1 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 5s.
Immunohistochemistry - PPP4R1 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded human liver using PPP4R1 antibody at dilution of 1:100 (40x lens).
Immunohistochemistry - PPP4R1 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded human stomach using PPP4R1 antibody at dilution of 1:100 (40x lens).
Immunoprecipitation - PPP4R1 Polyclonal Antibody
Immunoprecipitation analysis of 200ug extracts of 293T cells, using 3 ug PPP4R1 antibody . Western blot was performed from the immunoprecipitate using PPP4R1 antibody at a dilition of 1:1000.
-
Background
Regulatory subunit of serine/threonine-protein phosphatase 4. May play a role in regulation of cell division in renal glomeruli. The PPP4C-PPP4R1 PP4 complex may play a role in dephosphorylation and regulation of HDAC3.
Related Products / Services
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"