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Product Name
PPP2R5A antibody
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Description
PPP2R5A Rabbit Polyclonal antibody. Positive WB detected in human ileum tissue, human brain tissue, human heart tissue, human kidney tissue, human lung tissue. Positive IP detected in mouse brain tissue. Positive IHC detected in human pancreas cancer tissue. Observed molecular weight by Western-blot: 56 kDa
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Tested applications
ELISA, WB, IHC, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
B56A antibody; PP2A B subunit isoform B alpha antibody; PPP2R5A antibody; PR61A antibody; PR61alpha antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of PPP2R5A recombinant protein (Accession Number: NM_006243). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:1000-1:10000
IP: 1:200-1:2000
IHC: 1:20-1:200
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Validations
human ileum tissue were subjected to SDS PAGE followed by western blot with Catalog No:114105(PPP2R5A antibody) at dilution of 1:400
Immunohistochemical of paraffin-embedded human pancreas cancer using Catalog No:114105(PPP2R5A antibody) at dilution of 1:50 (under 10x lens)
IP Result of anti-PPP2R5A (IP:Catalog No:114105, 4ug; Detection:Catalog No:114105 1:500) with mouse brain tissue lysate 4000ug.
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Background
PPP2R5A(protein phosphatase 2, regulatory subunit B (B56), alpha isoform) is also named as PR61alpha and belongs to the phosphatase 2A regulatory subunit B56 family. B56 alpha subunit gene encodes a cytoplasmic phosphoprotein(PMID:7592815). The B regulatory subunit might modulate substrate selectivity and catalytic activity, and also might direct the localization of the catalytic enzyme to a particular subcellular compartment. This protein has 2 isoforms produced by alternative splicing with the molecular weight of 54 kDa and 50 kDa.
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