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Product Name
PPP2CA antibody
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Description
PPP2CA Rabbit Polyclonal antibody. Positive IHC detected in human liver cancer tissue. Positive WB detected in human liver tissue, Jurkat cells, MCF7 cells. Positive IP detected in Hela cells, Jurkat cells. Observed molecular weight by Western-blot: 36 kDa
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Tested applications
ELISA, WB, IHC, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
PP2A alpha antibody; PP2Ac antibody; PP2CA antibody; PPP2CA antibody; Replication protein C antibody; RP C antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of PPP2CA recombinant protein (Accession Number: NM_002715). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IP: 1:200-1:2000
IHC: 1:20-1:200
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Validations
human liver tissue were subjected to SDS PAGE followed by western blot with Catalog No:114095(PPP2CA antibody) at dilution of 1:300
IP result of anti-PPP2CA (Catalog No:114095 for IP and Detection) with HeLa cells.
Immunohistochemical of paraffin-embedded human liver cancer using Catalog No:114095(PPP2CA antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemical of paraffin-embedded human liver cancer using Catalog No:114095(PPP2CA antibody) at dilution of 1:50 (under 40x lens)
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Background
PPP2CA, also named as RP-C and PP2A-alpha, belongs to the PPP phosphatase family and PP-1 subfamily. PP2A can modulate the activity of phosphorylase B kinase casein kinase 2, mitogen-stimulated S6 kinase, and MAP-2 kinase. PP2A activity was modestly decreased in AML patients harboring C-KIT/WT.PP2A is a target for C-KIT/TKD and implicated the potential efficacy of therapies targeting PP2A in such AML in future.
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References
- Yang Y, Huang Q, Lu Y, Li X, Huang S. Reactivating PP2A by FTY720 as a novel therapy for AML with C-KIT tyrosine kinase domain mutation. Journal of cellular biochemistry. 113(4):1314-22. 2012.
- Kirchhefer U, Brekle C, Eskandar J. Cardiac function is regulated by B56α-mediated targeting of protein phosphatase 2A (PP2A) to contractile relevant substrates. The Journal of biological chemistry. 289(49):33862-73. 2014.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"